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Lipogro Bovine Cholesterol Concentrate

Product No.

Packaging Unit

RMB-001-001

1 Liter Bottle

RMB-001-004

100 ml Bottle

Product Description

Derived from the lipid fraction of adult bovine serum of Australian or New Zealand origin, RMBIO Lipogro® Bovine Cholesterol Concentrate is a high clarity, deep yellow, aqueous solution comprised of lipoproteins, including purified cholesterol and essential fatty acids. Lipogro® Bovine Cholesterol Concentrate can be used in mammalian cell culture to replace fetal bovine serum (FBS), reduce the amount of FBS used, or supplement existing serum free medias with increased cell growth, viability, and productivity. Lipogro® Bovine Cholesterol Concentrate is produced through a proprietary process with the advantages of larger batch sizes and increased lot-to-lot consistency, ensuring a high performing, superior product.

Applications

  • Bovine Cholesterol Concentrate is used to supplement media in serum containing, serum reduced, and serum free conditions in Myeloma (NSO, Sp20), Hybridoma, HEK293, and MDCK cell lines.
  • Also for production of veterinary vaccines, monoclonal antibodies, recombinant proteins, and live cellular therapies.

Lipogro® Bovine Cholesterol Concentrate should be stored at 2 - 8° C. Specific product requirements and typical values are given below:

Product Specifications

Parameter

Method

Specification

Total Cholesterol

Enzymatic Assay

9.1 - 11.0 g/L

Total Protein

Biuret Assay

< 2.0 g/dL

pH (7% Solution)

 

7.0 - 8.4

Endotoxin (Kinetic)

Lumulus Amebocyte Lysate

≤ 5.0 EU/mg

IgG

Radial Immunodiffusion

None Detected

Bioburden

USP

No Growth

Mycoplasma

 

None detected

Viral Agents

Tested in Accordance with 9 CFR 113.53 (c)

None detected

Heavy Metals

 

Upon Request

Lipogro Data on HEK293

Lipogro Data on NSO R9

 

Certificate of Suitability

Completion of Viral Inactivation Study