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Heat inactivated and gamma irradiated formula fed Bovine Calf Serum in a 1000 milliliter bottle.

Formula Fed Bovine Calf Serum - Heat inactivated and Gamma Irradiated

RMBIO produces high-quality, low hemoglobin, heat inactivated, gamma irradiated, US sourced calf serum for use in cell culture media. Bovine calf serum is often used as an alternative to Fetal Bovine Serum (FBS) as it’s available at a fraction of the price. This product is heat inactivated to help protect against growth inhibiting immune factors like serum complement that may be present in the serum. The serum is then gamma irradiated at an industry standard dose of 25-40kGy.
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Catalog Number : CSF-BHG
Purified from validated raw materials of the United States
Size
Price
Quantity
1 L
$165.00
Temporarily out of stock
500 ML
$103.00
Temporarily out of stock
50 ML
$30.00
 
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Product Details

RMBIO produces high-quality, low hemoglobin, heat inactivated, gamma irradiated, US sourced calf serum for use in cell culture media. Bovine calf serum is often used as an alternative to Fetal Bovine Serum (FBS) as it’s available at a fraction of the price. This product is heat inactivated to help protect against growth inhibiting immune factors like serum complement that may be present in the serum. The serum is then gamma irradiated at an industry standard dose of 25-40kGy.

  • Sterile 0.2 µm filtered
  • Average endotoxin level*: < 2 EU/ml (specification = ≤ 10.0 EU/ml)
  • Average hemoglobin level*: < 15 mg/dL (specification = < 30 mg/dL)
  • 9CFR tested
  • Heat inactivated at 56°C for 30 minutes
  • Gamma irradiated at a dose of 25-40kGy
  • For use in diagnostics or as cell culture medium
  • Manufactured at our GMP compliant and FDA registered facility
  • Large volume lot sizes available
  • Custom bottle sizes and containers available
  • Storage conditions: ≤ -10° C

*Data based on last 10 lots produced.

Heat Inactivation 101
Heat inactivation is an industry standard treatment that helps protect against growth inhibiting immune factors like serum complement that may be present in the serum. Serum complement can have undesirable impacts on immunological endpoints like detection of neutralizing antibodies, or on the proliferation of viruses. RMBIO manufactures heat inactivated bovine calf serum by raising the temperature of the serum to 56°C in a water bath and strictly maintaining the condition for 30 minutes.

Gamma Irradiation 101
Gamma irradiation is a physical inactivation approach used to achieve pathogen reduction in the serum. This mitigates risk from low-level contaminants that may not be detected through viral or mycoplasma testing. RMBIO serum is gamma irradiated via controlled exposure to Cobalt 60 at an industry standard dose of 25-40kGy. Most typical viral contaminants of bovine serum are readily inactivated at this irradiation range. Advantages of gamma irradiation include:

  • No potential for residual contaminants
  • Widely accepted by pharmaceutical and research industries
  • Accepted by regulatory agencies
  • Minimal effect on product performance
  • Effective virus inactivation

 

Formula Fed Bovine Calf Serum specific product test requirements and typical values are given below:


Parameter Method Specification
Total Protein Chemistry Analyzer As Reported
Osmolality By Osmometer 260 - 340 mOsm/Kg
pH pH Meter As Reported
Endotoxin LAL As Reported
Hemoglobin USP <90> ≤ 30 mg/dl
Sterility, Mycoplasma, and Viral Analysis 9CFR 113.26, 28, 53 Tested
Appearance Visual Clear Yellow Amber
Other Parameters Comprehensive Chemistry Testing As Reported
Heat Inactivation Product is heated at 56°C for 30 minutes to inactivate serum complement.
Gamma Irradiation 25-40kGy

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Certificate of Analysis

Lot: 20150818CS

Lot: 20160906CS